Archive

Recently, a new entomopathogenic nematode species Heterorhabditis gerrardi has been reported from Australia.  This nematode and its associated bacteria, Photorhabdus asymbiotica Kingscliff strain was identified using both morphological and molecular techniques.

Please read following paper on procedures used for identification of this new entomopathogenic nematode species.

Plichta, K.L., Joyce, S.A., Clarke, D., Waterfield, N. and Stock, S.P. 2009.  Heterorhabditis gerrardi n. sp (Nematoda: Heterorhabditidae): the hidden host of Photorhabdus asymbiotica (Enterobacteriaceae: gamma-Proteobacteria). Journal of Helminthology.83: 309-320.

A new species of Heterorhabditis brevicaudis and its symbiotic bacteria, Photorhabdus luminescens subsp. akhurstii has been reported for the first time from Taiwan.  This nematode was isolated from sandy coastal soils in moist bamboo forest.

Read following paper for more information on the methods used for identification of nematodes and its associated bacteria.

Hsieh, F.C., Tzeng, C.Y., Tseng, J.T., Tsai, Y.S., Meng, M.H. and Kao, S.S. 2009.  Isolation and Characterization of the Native Entomopathogenic Nematode, Heterorhabditis brevicaudis, and its Symbiotic Bacteria from Taiwan.  Current Microbiology. 58: 564-570.

An entomopathogenic nematode in a soil sample collected from a natural forest in Mato Grosso do Sul state, Brazil was described using both morphological and molecular characteristics as a new species “Steinernema brazilense (Rhabditida: Steinernematidae)” (Nguyen et al., 2010).

Reference:

Nguyen, K.B., Ginarte, C.M.A., Leite, L.G., dos Santos, J.M. and Harakava, R. 2010. Steinernema brazilense n. sp (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Mato Grosso, Brazil. Journal of Invertebrate Pathology. 103: 8-20.

Presence of an entomopathogenic nematode, Steinernema feltiae (Rhabditida: Steinernematidae) was recorded for first time in soil samples collected from grasslands and field crops in central part of Slovenia. Nematodes were isolated using Galleria-baiting technique (Bedding and Akhurst, 1975) and identified using molecular technique.

Read following literature for more information

Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110.

Laznik, Z., Toth, T., Lakatos, T., Vidrih, M. and Trdan, S. 2009.  First record of Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae) in Slovenia. Helminthologia. 46: 135-138.

An entomopathogenic nematode in the soil samples that collected from the northern part of the Yunnan province of China was described using both morphological and molecular characteristics as new species “Steinernema xueshannense“  (Mracek et al., 2009).

Reference:

Mracek, Z., Liu, Q., Nguyen, K. B. 2009. Steinernema xueshanense n. sp (Rhabditida, Steinernematidae), a new species of entomopathogenic nematode from the province of Yunnan, southeast Tibetan Mts., China. Journal of Invertebrate Pathology. 102: 69-78.

Presence of entomopathogenic nematode, Heterorhabditis megidis have been reported for the first time in the soil samples collected form Eastern Black Sea region of Turkey.  Nematodes were isolated using Galleria-baiting technique (Bedding and Akhurst, 1975) and identified using classical morphological (Poinar et al. 1987) and molecular techniques (Yilmaz et al., 2009).

Read following literature for more information

Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110

Poinar, G. O., Jr., T. Jackson, and M. Klein. 1987. Heterorhabditis megidis sp. n. (Heterorhabditidae: Rhabditida) parasitic in the Japanese beetle,Popillia japonica (Scarabaeidae: Coleoptera), in Ohio. Proceedings of the Helminthological Society of Washington 54:53-59.

Yilmaz, H., Waeyenberge, L., Demir, I., Moens, M., Demirbağ, Z. 2009. A new entomopathogenic nematode species for Turkey, Heterorhabditis megidis Poinar, Jackson & Klein 1987 (Rhabditida: Heterorhabditidae). Turkish Journal of Agriculture and Forestry. 33: 385-391.