A new species of entomopathogenic Steinernema nematode that isolated from southwest Bohemia, Czech Republic was identified and named as Steinernema poinari sp. n. (Nematoda : Steinernematidae) using both morphological and molecular techniques (Mráček et al., 2014). This new species was recovered from soil using Galleria baiting technique described by Bedding and Akhurst (1975).