quantitative real-time PCR (qPCR) technique

Use of real-time PCR in insect nematology /  by Ganpati Jagdale

Entomopathogenic nematodes and RT-PCR- nematodeinformation

Read following papers on the real-time PCR and Insect Nematology

Bae, S. and Kim, Y. 2003.   Lysozyme of the beet armyworm, Spodoptera exigua: activity induction and cDNA structure. Comparative Biochemistry and Physiology B-Biochemistry and Molecular Biology 135: 511-519.

Campos-Herrera R, El-Borai F.E., Stuart R.J., Graham J.H., DuncanL.W. 2011. Entomopathogenic nematodes, phoretic Paenibacillus spp., and the use of real time quantitative PCR to explore soil food webs inFlorida citrus groves. Journal Invertebrate Pathology 108:30-9.

Campos-Herrera, R., Johnson, E. G, El-Borai, F. E., Stuart, R. J., Graham, J. H. and Duncan, L. W.2011. Long-term stability of entomopathogenic nematode spatial patterns in soil as measured by sentinel insects and real-time PCR. Annals of Applied Biology 158: 55-68.

Ciche, T.A. and Sternberg, P.W. 2007.  Postembryonic RNAi in Heterorhabditis bacteriophora: a nematode insect parasite and host for insect pathogenic symbionts. BMC Developmental Biology 7, Article Number: 101.

Ji, D.J. and Kim, Y. 2004.   An entomopathogenic bacterium, Xenorhabdus nematophila, inhibits the expression of an antibacterial peptide, cecropin, of the beet armyworm, Spodoptera exigua. Journal of Insect Physiology 50: 489-496.

Park, D., Ciezki, K., van der Hoeven, R., Singh, S., Reimer, D., Bode, H.B. and Forst, S. 2009. Genetic analysis of xenocoumacin antibiotic production in the mutualistic bacterium Xenorhabdus nematophila. Molecular Microbiology 73: 938-949.

Pathak, E., El-Borai, F.E., Campos-Herrera, R., Johnson, E.G., Stuart, R.J., Graham, J.H. and Duncan, L.W. 2012.  Use of real-time PCR to discriminate parasitic and saprophagous behaviour by nematophagous fungi.  Fungal Biology 116: 563-573.

Shrestha, Y.K. and Lee, K.Y. 2012. Oral toxicity of Photorhabdus culture media on gene expression of the adult sweetpotato whitefly, Bemisia tabaci. Journal of Invertebrate Pathology 109: 91-96.

Son, Y. and Kim, Y. 2011.  Immunosuppression induced by entomopathogens is rescued by addition of apolipophorin III in the diamondback moth, Plutella xylostella. Journal of Invertebrate Pathology 106: 217-222.

Song, C.J., Seo, S., Shrestha, S. and Kim, Y.  2011. Bacterial Metabolites of an Entomopathogenic bacterium, Xenorhabdus nematophila, inhibit a catalytic activity of phenoloxidase of the diamondback moth, Plutella xylostella. Journal of Microbiology and Biotechnology 21: 317-322.

Torr, P., Spiridonov, S.E., Heritage, S. and Wilson, M.J. 2007. Habitat associations of two entomopathogenic nematodes: a quantitative study using real-time quantitative polymerase chain reactions. Journal of Animal Ecology 76: 238-245.

Entomopathogenic nematode identification with a quantitative real-time PCR (qPCR) /  by Ganpati Jagdale

Entomopathogenic nematodes and qPCR Quantitative real-time PCR (qPCR) technique can be used for the identification of entomopathogenic nematodes in the both Heterorhabditidae and Steinernematodae families directly from soil samples.

Species specific primers and TaqMan (R) probes from the ITS rDNA region for the EPNs were used for the identification of four species of entomopathogenic nematodes including Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae and Steinernema scapterisci (Campos-Herrera et al., 2011).

A publication on indentification of entomopathogenic nematodes using quantitative real-time PCR (qPCR) technique.

Campos-Herrera, R., El-Borai, F.E., Stuart, R.J., Graham, J.H. and Duncan, L.W. 2011.   Entomopathogenic nematodes, phoretic Paenibacillus spp., and the use of real time quantitative PCR to explore soil food webs in Florida citrus groves. Journal of Invertebrate Pathology 108: 30-39.